CRISPR

CRISPR1-vrk1

ID

ZDB-CRISPR-230607-3

Name

CRISPR1-vrk1

Previous Names

None

Target

vrk1 (1)

Sequence

5' - CTGTCAGGACTTCTCCAGAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end. This CRISPR also appears to target a processed pseudogene ENSDARG00000091657.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
oi6	vrk1
zf4093	vrk1

Expression

Gene expression in Wild Types + CRISPR1-vrk1

No data available

Phenotype

Phenotype resulting from CRISPR1-vrk1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-vrk1

Phenotype	Fish	Conditions	Figures
forebrain decreased diameter, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 2 from Carrasco Apolinario et al., 2023
whole organism vrk1 expression decreased amount, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 1 from Carrasco Apolinario et al., 2023
midbrain decreased diameter, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 2 from Carrasco Apolinario et al., 2023
whole organism vrk2 expression increased amount, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 1 from Carrasco Apolinario et al., 2023
behavioral fear response decreased process quality, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 3 from Carrasco Apolinario et al., 2023
radial glial cell cell population proliferation decreased process quality, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 4 from Carrasco Apolinario et al., 2023
telencephalon neuron decreased amount, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 2 from Carrasco Apolinario et al., 2023
forebrain nuclear envelope structure, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 4 from Carrasco Apolinario et al., 2023
swimming behavior decreased process quality, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 3 from Carrasco Apolinario et al., 2023
midbrain decreased area, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 2 from Carrasco Apolinario et al., 2023
whole organism decreased length, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 1 from Carrasco Apolinario et al., 2023
cerebellum decreased diameter, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 2 from Carrasco Apolinario et al., 2023
brain decreased area, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 2 from Carrasco Apolinario et al., 2023
radial glial cell decreased amount, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 4 from Carrasco Apolinario et al., 2023
forebrain heterochromatin increased distribution, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 4 from Carrasco Apolinario et al., 2023
cerebellum decreased area, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 2 from Carrasco Apolinario et al., 2023
brain dopamine decreased amount, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 3 from Carrasco Apolinario et al., 2023
forebrain decreased area, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 2 from Carrasco Apolinario et al., 2023
testis decreased diameter, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. S2 from Carrasco Apolinario et al., 2023
telencephalon heterochromatin formation increased process quality, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 4 from Carrasco Apolinario et al., 2023
head decreased diameter, abnormal	vrk1^{zf4093/zf4093} (AB)	standard conditions	Fig. 1 from Carrasco Apolinario et al., 2023
testis has fewer parts of type sperm, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 4 from Apridita Sebastian et al., 2022
brain size, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022
dorsal telencephalon generation of neurons process quality, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022
dorsal telencephalon has number of radial glial cell, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022
spermatogenesis occurrence, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 4 from Apridita Sebastian et al., 2022
brain nuclear envelope morphology, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022
male organism male sterile, abnormal	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 4 from Apridita Sebastian et al., 2022
testis size, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 4 from Apridita Sebastian et al., 2022
dorsal telencephalon neural precursor cell proliferation process quality, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022
brain nuclear envelope organization process quality, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022

Citations