CRISPR

CRISPR2-vps28

ID

ZDB-CRISPR-230607-4

Name

CRISPR2-vps28

Previous Names

None

Target

vps28 (1)

Sequence

5' - GGACCGACCGATCACCATTAAGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3789	vps28

Expression

Gene expression in Wild Types + CRISPR2-vps28

No data available

Phenotype

Phenotype resulting from CRISPR2-vps28

No data available

Phenotype of all Fish created by or utilizing CRISPR2-vps28

Phenotype	Fish	Conditions	Figures
head vasculature hemorrhagic, abnormal	vps28^{zf3789/zf3789}	standard conditions	Fig. 1 from Dong et al., 2022
endothelial blood brain barrier increased permeability, abnormal	vps28^{zf3789/zf3789}	standard conditions	Fig. 1 from Dong et al., 2022
brain vasculature multivesicular body decreased amount, abnormal	vps28^{zf3789/zf3789}	standard conditions	Fig. 4 from Dong et al., 2022
brain vasculature endosome decreased amount, abnormal	vps28^{zf3789/zf3789}	standard conditions	Fig. 4 from Dong et al., 2022
primordial hindbrain channel central artery EGFP expression spatial pattern, abnormal	vps28^{zf3789/zf3789}; s843Tg/s843Tg	standard conditions	Fig. 5 , Fig. 6 from Dong et al., 2022
primordial hindbrain channel central artery EGFP expression decreased amount, abnormal	vps28^{zf3789/zf3789}; s843Tg/s843Tg	standard conditions	Fig. 5 from Dong et al., 2022
primordial hindbrain channel extracellular exosome Ab5-vegfa labeling decreased amount, abnormal	vps28^{zf3789/zf3789}; s843Tg/s843Tg	standard conditions	Fig. 6 from Dong et al., 2022
primordial hindbrain channel central artery EGFP expression decreased distribution, abnormal	vps28^{zf3789/zf3789}; s843Tg/s843Tg	standard conditions	Fig. 5 from Dong et al., 2022
endothelial blood brain barrier increased permeability, abnormal	vps28^{zf3789/zf3789}; s843Tg/s843Tg	standard conditions	Fig. 6 from Dong et al., 2022
central artery vascular endothelial growth factor signaling pathway decreased efficacy, abnormal	vps28^{zf3789/zf3789}; s843Tg/s843Tg	standard conditions	Fig. 6 from Dong et al., 2022
blood DsRed expression spatial pattern, abnormal	vps28^{zf3789/zf3789}; s843Tg/s843Tg; sd2Tg/sd2Tg	standard conditions	Fig. 1 from Dong et al., 2022
central artery EGFP expression spatial pattern, abnormal	vps28^{zf3789/zf3789}; s843Tg/s843Tg; sd2Tg/sd2Tg	standard conditions	Fig. 1 from Dong et al., 2022
artery EGFP expression spatial pattern, abnormal	vps28^{zf3789/zf3789}; s843Tg/s843Tg; sd2Tg/sd2Tg	standard conditions	Fig. 1 from Dong et al., 2022
central artery vasculature development decreased process quality, abnormal	vps28^{zf3789/zf3789}; s843Tg/s843Tg; sd2Tg/sd2Tg	standard conditions	Fig. 1 from Dong et al., 2022

Citations