CRISPR

CRISPR3-trrap

ID
ZDB-CRISPR-230828-15
Name
CRISPR3-trrap
Previous Names
None
Target
Sequence
5' - AGACATGAACGCTCCTGTCA - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "GGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
uy45 trrap
Expression
Gene expression in Wild Types + CRISPR3-trrap
No data available
Phenotype
Phenotype resulting from CRISPR3-trrap
No data available
Phenotype of all Fish created by or utilizing CRISPR3-trrap
Phenotype Fish Conditions Figures
ceratobranchial 5 tooth pitx2 expression decreased distribution, abnormal trrapuy45/uy45 standard conditions Figure 6 with image from Suzuki et al., 2021
eye decreased diameter, abnormal trrapuy45/uy45 standard conditions Figure 2 with image from Suzuki et al., 2021
ceratobranchial 5 tooth dlx2b expression decreased distribution, abnormal trrapuy45/uy45 standard conditions Figure 6 with image from Suzuki et al., 2021
pharyngeal arch ventral region dlx2a expression decreased distribution, abnormal trrapuy45/uy45 standard conditions Figure 5 with image from Suzuki et al., 2021
pharyngeal arch ventral region dlx3b expression decreased distribution, abnormal trrapuy45/uy45 standard conditions Figure 5 with image from Suzuki et al., 2021
ceratobranchial 5 tooth ossification decreased process quality, abnormal trrapuy45/uy45 standard conditions Figure 4 with image from Suzuki et al., 2021
ceratohyal cartilage increased angle to ceratohyal cartilage, abnormal trrapuy45/uy45 standard conditions Figure 4 with image from Suzuki et al., 2021
ceratohyal cartilage decreased length, abnormal trrapuy45/uy45 standard conditions Figure 4 with image from Suzuki et al., 2021
pharyngeal arch ventral region nkx2.3 expression decreased distribution, abnormal trrapuy45/uy45 standard conditions Figure 5 with image from Suzuki et al., 2021
head decreased size, abnormal trrapuy45/uy45 standard conditions Figure 3 with image from Suzuki et al., 2021
cranium ossification decreased process quality, abnormal trrapuy45/uy45 standard conditions Figure 4 with image from Suzuki et al., 2021
Citations
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