CRISPR

CRISPR1-f3a

ID

ZDB-CRISPR-240328-16

Name

CRISPR1-f3a

Previous Names

None

Target

f3a (1)

Sequence

5' - GGATACGCTTGTATTGGCGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first two "G"s were added.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
mi4009	f3a

Expression

Gene expression in Wild Types + CRISPR1-f3a

No data available

Phenotype

Phenotype resulting from CRISPR1-f3a

No data available

Phenotype of all Fish created by or utilizing CRISPR1-f3a

Phenotype	Fish	Conditions	Figures
blood coagulation delayed, abnormal	f3a^{mi4009/mi4009}	light ablation: posterior cardinal vein	Fig 4 from Grzegorski et al., 2022
vein blood circulation decreased rate, abnormal	f3a^{mi4009/mi4009}	light ablation: posterior cardinal vein	Fig 4 from Grzegorski et al., 2022
whole organism f3b expression decreased amount, abnormal	f3a^{mi4009/mi4009}	standard conditions	Fig 1 from Grzegorski et al., 2022
whole organism f3b expression decreased amount, abnormal	f3a^mi4009/+	standard conditions	Fig 1 from Grzegorski et al., 2022
artery blood circulation decreased rate, abnormal	f3a^mi4009/+; f3b^{mi4010/mi4010}	light ablation: dorsal aorta	Fig 4 from Grzegorski et al., 2022
blood coagulation delayed, abnormal	f3a^mi4009/+; f3b^{mi4010/mi4010}	light ablation: dorsal aorta	Fig 4 from Grzegorski et al., 2022
blood coagulation delayed, abnormal	f3a^{mi4009/mi4009}; f3b^mi4010/+	light ablation: posterior cardinal vein	Fig 4 from Grzegorski et al., 2022
vein blood circulation decreased rate, abnormal	f3a^{mi4009/mi4009}; f3b^mi4010/+	light ablation: posterior cardinal vein	Fig 4 from Grzegorski et al., 2022
blood coagulation absent process, abnormal	f3a^{mi4009/mi4009}; f3b^{mi4010/mi4010}	light ablation: posterior cardinal vein	Fig 4 from Grzegorski et al., 2022
artery blood circulation decreased rate, abnormal	f3a^{mi4009/mi4009}; f3b^{mi4010/mi4010}	light ablation: dorsal aorta	Fig 4 from Grzegorski et al., 2022
pericardium blood cell increased amount, abnormal	f3a^{mi4009/mi4009}; f3b^{mi4010/mi4010}	chemical treatment by environment: (R)-adrenaline, chemical treatment by environment: cortisol	Fig 7 from Grzegorski et al., 2022
blood coagulation absent process, abnormal	f3a^{mi4009/mi4009}; f3b^{mi4010/mi4010}	light ablation: dorsal aorta	Fig 4 from Grzegorski et al., 2022
whole organism dead, abnormal	f3a^{mi4009/mi4009}; f3b^{mi4010/mi4010}	standard conditions	Fig 2 from Grzegorski et al., 2022
blood coagulation delayed, abnormal	f3a^{mi4009/mi4009}; f3b^{mi4010/mi4010}	light ablation: dorsal aorta	Fig 4 from Grzegorski et al., 2022
pericardium hemorrhagic, abnormal	f3a^{mi4009/mi4009}; f3b^{mi4010/mi4010}	standard conditions	Fig 2 from Grzegorski et al., 2022
head hemorrhagic, abnormal	f3a^{mi4009/mi4009}; f3b^{mi4010/mi4010}	standard conditions	Fig 2 from Grzegorski et al., 2022
vein blood circulation decreased rate, abnormal	f3a^{mi4009/mi4009}; f3b^{mi4010/mi4010}	light ablation: posterior cardinal vein	Fig 4 from Grzegorski et al., 2022
artery blood circulation decreased rate, abnormal	f3a^{mi4009/mi4009}; f9b^mi4013/+	light ablation: dorsal aorta	Fig 5 from Grzegorski et al., 2022
blood coagulation delayed, abnormal	f3a^{mi4009/mi4009}; f9b^mi4013/+	light ablation: dorsal aorta	Fig 5 from Grzegorski et al., 2022
blood coagulation delayed, ameliorated	f3a^{mi4009/mi4009}; f9b^{mi4013/mi4013}	light ablation: dorsal aorta	Fig 5 from Grzegorski et al., 2022
artery blood circulation decreased rate, ameliorated	f3a^{mi4009/mi4009}; f9b^{mi4013/mi4013}	light ablation: dorsal aorta	Fig 5 from Grzegorski et al., 2022
blood coagulation delayed, abnormal	f3a^{mi4009/mi4009}; f9b^{mi4013/mi4013}	light ablation: posterior cardinal vein	Fig 5 from Grzegorski et al., 2022
vein blood circulation decreased rate, abnormal	f3a^{mi4009/mi4009}; f9b^{mi4013/mi4013}	light ablation: posterior cardinal vein	Fig 5 from Grzegorski et al., 2022
blood coagulation delayed, abnormal	f3a^{mi4009/mi4009}; serpinc1^{mi4018/mi4018}	standard conditions	Fig 6 from Grzegorski et al., 2022
whole organism dead, abnormal	f3a^{mi4009/mi4009}; serpinc1^{mi4018/mi4018}; f3b^{mi4010/mi4010}	standard conditions	Fig 6 from Grzegorski et al., 2022
blood coagulation absent process, abnormal	f3a^{mi4009/mi4009}; serpinc1^{mi4018/mi4018}; f3b^{mi4010/mi4010}	standard conditions	Fig 6 from Grzegorski et al., 2022

Citations