Fig. 6 Spatial requirement for pou2. Animal views, if not noted otherwise. (A-M) An asterisk indicates the shield. (F',H,J,L,N) Upper half with and lower half without phase contrast to show the shield, or expression, respectively. (A'-M') Bright-field images of injected living embryos were merged with UV images taken with the same adjustment. (A''-M'') UV images show EGFP expression, corresponding to A'-M'. (A-J) pou2 mRNA injection into ventro-marginal blastomeres of MZspg embryos at early cleavage stages can restore bmp4 expression. (B') bmp4 expression at beginning of shield stage in the wild-type embryo. (K-N) pou2 mRNA injection into dorsal or animal blastomeres of MZspg embryos at early cleavage stages cannot restore bmp4 expression.
                
                    
                        Image
                    
                    
                
                
            
        
        
    
        
            
            
        
        
    
    
    
                
                    
                        Figure Caption
                    
                    
                
                
            
        
        
    
        
            
            
                
                    
                        Figure Data
                    
                    
                
                
            
        
        
    
        
            
            
 
    
    
        
    
        
        
    
    
    
                
                    
                        Acknowledgments
                    
                    
                
                
            
        
        
    
        
            
            
                
                    
                        
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
      Full text @ Development