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Figure Caption
Fig. 5 Immunohistochemical identification of Wnt-responsive and Lef1-expressing cells in the adult hypothalamus. (A) GFP antibody staining on a sagittal section through the midline of a TOP:dGFP adult brain. Specific expression of GFP is observed in the optic tectum (white box) and the periventricular hypothalamus (red box). (B–D) Twenty-five-micron hypothalamic cross sections immunostained for the markers listed in each panel. GFP-positive cells do not express HuC/D, PCNA, or GFAP. Scale bars: (A) 300 μm; (B) 100 μm.
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