Fig. S1
- ID
- ZDB-IMAGE-130211-15
- Publication
- Sarris et al., 2012 - Inflammatory Chemokines Direct and Restrict Leukocyte Migration within Live Tissues as Glycan-Bound Gradients
- All Figures
- Figures for Sarris et al., 2012
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Fig. S1
zcxcl8 Is Upregulated upon Infection, and zcxcl8 Knockdown Does Not Affect Larval Development or Neutrophil Distribution (Related to Figure 1)
(A and B) qPCR analysis of zcxcl8-chr 1 (A and B), zcxcl8-chr 7a (A), zcxcl8-chr 7b (A) mRNA expression 0.5, 2 and 4 hours post injection (hpi) of E.coli. NI, non injected. Data are normalized to ef1a expression. n=3 qPCR samples. Error bars: SEM. Data in A and B are from two independent experiments, each with pools of 15-20 larvae per condition.
(C) Bright field and wide-field fluorescence images of whole Tg(mpx:gfp) larvae at 2.5 dpf treated or not (control) with splice-blocking (sMO) or combined splice and translation-blocking (sMO+tMO) zCxcl8 morpholinos. E, eye. Y, yolk sac.
(D) Flow cytometry of whole Tg(mpx:gfp) larvae, treated or not with splice-blocking (sMO) or combined splice and translation-blocking (sMO+tMO) zCxcl8 morpholinos. GFP+ neutrophils are boxed (green) and their percentage over the total cell number is shown. Pools of 15 larvae. Representative of four independent experiments.