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Fig. 4 Expression of melanoblast markers is absent from nacre-/- neural crest. Whole-mount in situ hybridization was performed on 23 hpf embryos with probes for trp2(A) and c-kit (B). Expression of trp2 is strong in the eye of nacre mutants (arrow) but absent from the neural crest (arrowheads). Likewise, nacre-/- embryos show normal expression of the receptor tyrosine kinase c-kit in the intermediate cell mass (arrow) and elsewhere but little or no expression in presumptive melanoblasts (arrowheads). Scale bar, 250 μm.
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