|
Fig. 5 Effect of retinoic acid treatment on wild-type and syu pectoral fin buds. (A-F) retinoic acid-treated embryos. (A) Embryo at 52 hours; (B-F) whole-mount in situs of embryos at 32 hours of development. (A,B,C,E) Wild-type embryos treated with retinoic acid; (D,F) syu embryos treated with retinoic acid. (B) shh RNA; (C,D) hoxd-10 RNA; (E,F) hoxd-11 RNA. The pectoral fin buds are elongated and located next to the heavily pigmented anterior end of both wild-type and syu embryos treated with retinoic acid (arrows in A to E). Note also that the posterior markers shh and hoxd-11 are expressed throughout the fin bud mesenchyme in wild-type embryos (arrows in B and E), but not in syu embryos treated with retinoic acid, which instead show a patch of hoxd-11 expression at the posterior margin of the fin bud (arrow in F).