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Figure 4

ID: ZDB-IMAGE-190723-476
Publication: Dohaku et al., 2019 - Tracing of Afferent Connections in the Zebrafish Cerebellum Using Recombinant Rabies Virus
All Figures: Figures for Dohaku et al., 2019

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Figure Caption

Figure 4

Transgenic lines for tracing GC afferents. (A) Schematic drawing of the Tol2 plasmids used to express TVA-mCherry or RVG. The cbln12 promoter was used to express transgenes in GCs. pBH was used to identify G-expressing fish. (B,C) Immunostaining of sagittal sections of the adult Tg(cbln12:TVA-mCherry) cerebellum with anti-mCherry (magenta) and anti-Neurod1 (green) antibodies. (C) High-magnification images of the box in (B). TVA-mCherry (Ba,Ca), Neurod1 (Bb,Cb), and merged images (B,C) are shown. Note that TVA-mCherry was located on the cell membrane of Neurod1⁺ GCs (C). Scale bars: (B) 50 μm, and (C) 5 μm.

Figure Data

Constructs: Tg(cbln12:Tva-mCherry) , Tg(cbln12:EnvA,myl7:mCherry)

Acknowledgments

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