FIGURE 8

FIGURE 8

Rab28 interacts with multiple phototransduction proteins in the zebrafish eye. (A) Schematic of experimental workflow for Co-IP/MS of eGFP-Rab28 (B) Western blotting with an anti-GFP antibody, showing eGFP-Rab28 in whole eye lysate and elute after immunoprecipitation with anti-GFP beads. An untagged eGFP only control is also shown. (C) Venn diagram showing the number and overlap of significantly enriched (vs. GFP-only control) interacting proteins identified for each variant. (D) Pie charts showing gene ontology terms for proteins identified by mass-spectrometry following co-immunoprecipitation, which demonstrate a statistically significant interaction with any of the three variants. (E) Western blot of whole adult zebrafish eye lysate with an anti-PDE6D antibody, before and after IP of eGFP-Rab28. PDE6D band at 15 kDa mark (PDE6D mass: 17.4 kDa). Extra bands in the lysates are either post-translationally modified PDE6D, degradation products or non-specific binding by the antibody. IPs either received no treatment, GTPγS or GDP. eGFP only control is also shown.