Figure 7

Figure 7

MCU-mediated mitochondrial Ca²⁺ uptake does not modulate the photoresponses in Nckx1^-/- mice. (A, B) Flash response families of dark adapted Nckx1^-/- iCre⁺ (Nckx1^-/- control; A) and Nckx1^-/-Mcu^f/f iCre⁺ double knockout (Nckx1^-/- Rod Mcu^-/-; B) mice from transretinal ERG recordings. Scotopic a-wave responses were recorded by a series of test flashes (1 ms in duration) with intensities (in photons/µm²) 1, 3.5, 10.2 (red traces), 35.4, 117. (C) Averaged rod responses (Mean ± SEM) from Nckx1^-/- control (black; n = 10) and Nckx1^-/- Rod Mcu^-/- mice (red; n = 9) plotted as a function of flash intensity show a substantial reduction of the response amplitude in the double knockouts as compared to the controls. However, the sensitivity of the rods (estimated their normalized intensity response functions; Inset) remained unchanged between Nckx1^-/- control (black) and Nckx1^-/- Rod Mcu^-/- (red) mice. (D) The kinetics of the dim flash response were not affected in the Nckx1^-/- Rod Mcu^-/- mice (n = 9); red trace) as compared to Nckx1^-/- controls (n = 10; black trace).