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Figure 6—figure supplement 2.

ID: ZDB-IMAGE-201223-36
Publication: Chin et al., 2020 - Cohesin mutations are synthetic lethal with stimulation of WNT signaling
All Figures: Figures for Chin et al., 2020

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Figure Caption

Figure 6—figure supplement 2. Enhanced sensitivity of cohesin-mutant CMK cells to Wnt stimulation. Enhanced sensitivity of cohesin-mutant CMK cells to Wnt stimulation.

(A) Sanger sequencing shows successful CRISPR-CAS9 editing introducing the STAG2 R614* C > T mutation site into CMK cells. (B) Reduced STAG2 mRNA and (C) protein in STAG2-/- CMK cells. (D) Heatmap showing log2 RPKM values of 402 genes upregulated only in STAG2 mutant cells (FDR ≤ 0.05) following 4 hr of WNT3A treatment. (E) GSEA analyses of the 402 upregulated genes. (F) Heatmap showing log2 RPKM values of 171 genes downregulated only in STAG2 mutant cells (FDR ≤ 0.05) following 4 hr of WNT3A treatment. (G) GSEA analyses of the 171 genes significantly downregulated only in the STAG2 mutant line. Gene lists are provided in Figure 6—source data 1.

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