IMAGE

Figure 6—figure supplement 1.

ID: ZDB-IMAGE-210119-20
Publication: Ohta et al., 2020 - Emx2 regulates hair cell rearrangement but not positional identity within neuromasts
All Figures: Figures for Ohta et al., 2020

Image

Figure Caption

Figure 6—figure supplement 1. Measurements of signal intensity and length of the apical protrusion using ImageJ Fiji.

(A) A selected maximum intensity projection image was converted into an 8-bit gray scale image. (B) The gray scale image in (A) was converted to Rainbow RBG color mode to visualize the bright green color of the accumulated YFP-tubulin within the apical protrusion. (C) Based on the signal intensity, the region of interest (ROI, yellow outline) was automatically defined by using the wand selection tool of ImageJ Fiji and the intensity measured. (D) The ROI was converted into a mask image (white area). A blue line was drawn across the top of the two hair cell (HC) nuclei, and a pink line joining the tip of the apical protrusion to the blue line at right angle indicates the length of the apical protrusion.

Acknowledgments

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