FIGURE 6

FIGURE 6

Perturbation of the Papp-aa-Igfbp-a-IGF signaling loop rescues the body edema and premature death in stc1a^{– /–} fish. (A,C) Gross morphology of fish of the indicated genotypes at the indicated stages. Lateral views with anterior to the left and dorsal up. Scale bar = 0.5 mm. (B,D) Survival curves. Embryos were raised in E3 embryo medium. Dead embryos were collected daily and genotyped individually. The survival curves of indicated genotypes and the total fish numbers are shown. P < 0.0001 by log-rank test. (E–J) Progeny of stc1a (+ 17)^+/– intercrosses were raised in E3 embryo media containing DMSO (E,F), 0.3 μM BMS-754807 (G,H) or 5 μM Rapamycin (I,J) starting from 3 dpf until the indicated time. Representative morphological views of the indicated genotypes at the indicated stages are shown in (E,G,I). Scale bar = 0.5 mm. P < 0.0001 by log-rank test. Dead fish were collected daily and genotyped individually. The survival curves of the indicated genotypes and the total fish numbers are shown in (F,H,J).