IMAGE

Figure 6

ID

ZDB-IMAGE-211002-7

Genes

appa
appb

Antibodies

Publication

Chebli et al., 2021 - The localization of amyloid precursor protein to ependymal cilia in vertebrates and its role in ciliogenesis and brain development in zebrafish

All Figures

Figures for Chebli et al., 2021

Image

Figure Caption

Figure 6

Generation of appa^−/− and analysis of appa^−/−appb^−/− double mutant zebrafish. (A) Schematic outline of the appa gene with exons (black box) and UTR regions (white box). sgRNA used to target exon 2 with protospacer adjacent motif (PAM) in red and the sgRNA target sequence underlined. (B) Sanger sequencing chromatogram of the exon 2 region targeted in wild-type zebrafish. The appa^−/− mutant sequence is given below with dash indicating deleted nucleotides. (C) Schematic drawing of the wild-type Appa protein (738 aa) with epitopes of antibodies (dotted squares) used above and the hypothetical truncated Appa (109 aa) protein produced in appa mutant below. (D) qPCR quantification of appa and appb mRNA levels in wild-type and appa^−/−appb^−/− mutants at 24 hpf. (E) Western blot of 3 dpf whole larvae zebrafish with antibodies against 22C11 and App (Y188). Alpha-tubulin is used as loading control. Blots cropped from the same original gel and grouped. Quantification of band intensity are shown relative to control. Data are reported as mean ± SD. ** ρ < 0.05, **** ρ < 0.001. qPCR n = 5, WB n = 3. SP signal peptide, E1 extracellular domain, ED extension domain, AcD acidic domain, E2 extracellular domain 2, JMR juxtamembrane region, Aβ amyloid beta, AICD amyloid intracellular domain.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.