ZFIN Image: Li et al., 2022, Fig. 6

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Figure Caption

Fig. 6

E2 enhances neutrophil apoptosis through suppressing the expression of hif1? and c-myb under physiological conditions.

A E2 decreased neutrophils in AB zebrafish embryos. (t-test, ***p?<?0.001, n?>?20). B May?Grunwald?Giemsa staining of whole KM blood cells in 6-month-old AB zebrafish after 4 days of E2 treatment (t-test, ***p?<?0.001, n?=?12). Red arrowheads, blue asterisks, black arrowheads and yellow lightning indicates neutrophils, precursors, lymphocytes and macrophages, respectively. C Staining of hif1? with antibody with or without E2 treatment. D E2 exposure decreased c-myb in the CHT, as determined by WISH. E qPCR quantification of decreased c-myb expression in lyz:Dsred⁺ cells by E2 (t-test, mean?�?SEM. ***p?<?0.001, **p?<?0.01, ^*p?<?0.05, n?>?20). F Effect of E2 on neutrophil proliferation in AB zebrafish embryos. (one-way ANOVA (LSD). ns, no significance, n?>?10). G E2 promotes the apoptosis of myeloid lineage cells in AB zebrafish embryos (one-way ANOVA (LSD) **p?<?0.01. n?>?10).

Acknowledgments

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