Figure 3

Figure 3

Visualization of usherin on retinal sections of wild-type, ush2a^rmc1, ush2a^Δexon30-31, and ush2a^Δexon39-40 zebrafish

(A) Retinal cryosections of wild-type, ush2a^rmc1, ush2a^Δexon30-31, and ush2a^Δexon39-40 larvae (5 dpf) were stained with antibodies directed against usherin (red) and centrin (green). Nuclei are counterstained with DAPI (blue). No usherin signal was detected in the retina of ush2a^rmc1 zebrafish, whereas in the retinas of wild-type, ush2a^Δexon30-31, and ush2a^Δexon39-40 larvae, usherin was present at the photoreceptor periciliary region, in close proximity to centrin. Scale bar, 10 μm. CC, connecting cilium; IS, inner segment; ONL, outer nuclear layer; OS, outer segment. (B) Quantification of anti-usherin signal intensity at the periciliary region. Individual data points represent the mean fluorescence intensity of anti-usherin staining at the periciliary region of all photoreceptors of a single, central section of one larval zebrafish eye. Horizonal bars depict the mean signal intensity within a genotype (n = 25–28 eyes). Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test. ∗p ≤ 0.05. ∗∗∗∗p ≤ 0.0001.