ZFIN Image: Schellens et al., 2023, Figure 4

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Figure 4

Visualization of Adgrv1 in retinal sections of wild-type, ush2a^rmc1, ush2a^Δexon30-31, and ush2a^Δexon39-40 zebrafish

(A) Retinal cryosections of wild-type, ush2a^rmc1, ush2a^Δexon30-31, and ush2a^Δexon39-40 larvae (5 dpf) stained with antibodies directed against Adgrv1 (red) and centrin (green). Nuclei are counterstained with DAPI (blue). A reduced Adgrv1 signal was detected in ush2a^rmc1 zebrafish retinas, whereas in the retinas of wild-type, ush2a^Δexon30-31, and ush2a^Δexon39-40 larvae, usherin was present adjacent to the centrin immunoreactivity. Scale bar, 10 μm. CC, connecting cilium; IS, inner segment; ONL, outer nuclear layer; OS: outer segment. (B) Quantification of anti-Adgrv1 signal intensity at the periciliary region. Individual data points represent the mean fluorescence intensity of anti-Adgrv1 staining at the periciliary region of all photoreceptors of a single, central section of one larval zebrafish eye. Horizonal bars depict the mean signal intensity within a genotype (n = 26–28 eyes). Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test. ∗∗∗∗p ≤ 0.0001.

Acknowledgments

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