Figure 7

Figure 7 Demethylation activity is required for FTO to regulate EC features

(A) m⁶A dot blot assay of global m⁶A abundance in HUVECs transduced with L-EV, L-FTO^WT or L-FTO^MU using 800 or 400 ng total RNAs. MB staining is used as a loading control. n = 3 per group. (B) Immunoblotting of FTO in HUVECs transduced with L-EV, L-FTO^WT or L-FTO^MU. GAPDH is used as an internal control. n = 3 per group. (C) Flow cytometric analyses of cell cycle process in HUVECs transduced with L-EV, L-FTO^WT or L-FTO^MU. n = 3 per group. (D) EdU assay on HUVECs transduced with indicated lentivirus. n = 3 per group. Scale bar: 60 µm. (E) Scratch test on HUVECs transduced with L-EV, L-FTO^WT or L-FTO^MU. n = 3 per group. Scale bar: 100 µm. (F) Transwell migration assay on HUVECs transduced with indicated lentivirus. n = 3 per group. Scale bar: 50 µm. (G) Tube formation analyses on HUVECs transduced with L-EV, L-FTO^WT or L-FTO^MU. n = 3 per group. Scale bar: 100 µm. Data information: Data represent different numbers (n) of biological replicates. Data are shown as mean ± SEM. One-way ANOVA followed by Bonferroni’s test is used. NS: not significant (p > 0.05); *p < 0.05; and ***p < 0.001. Source data are available online for this figure.