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Fig. 1 Zebrafish ASS1 homologue is expressed in the midbrain-hindbrain boundary (MHB) and the optic tectum (OT) during early development. (A) Endogenous mRNA expression of ASS1 during zebrafish development (50% epiboly to 5 dpf). Expression of ASS1 showed a monophasic expression pattern with increasing concentrations during neurulation peaking at 1 dpf. (B, C) Localization of mRNA coding for ASS1 in the CNS of zebrafish larvae as visualized by ISH. Images show the embryonic heads at stages indicated. Left columns of each row show lateral view with anterior to the left, and right columns illustrate top view with anterior to the left. ASS1 was expressed in the mes- and diencephalon mainly confined to the MHB (arrow head) and the OT (asterisk) at 1 dpf (B). At 3 dpf, ASS1 expression was retained and even more confined to a distinct zone in the OT (asterisk; C). Data are expressed as mean +/− SD in fold-change (A; whole body lysates, N = 3 with 50 larvae per group and experiment).