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Fig. 5 Loss of EHD2 promotes dysmorphic sprouting in zebrafish blood vessels. (A,B) DNA sequence alignments of EHD2a or EHD2b CRISPR injected embryos to a wild-type (WT) sequence (n = 3). (C) Message RNA levels of either EHD2a or EHD2b relative to a scramble control after CRISPR injections. RNA was collected at 72 h post fertilization (hpf). 10–20 fish were pooled per repeat. N, number of repeats. (D) Representative images of intersomtic blood vessels (ISVs) on vascular reporter tg(kdrl:GFP)+/+ background at 48 hpf injected with indicated CRISPR guides. Yellow arrowheads denote abnormal vascular growth. Red dashed box denotes area of higher magnification. Larvae cartoon denotes location of imaging. (E) Quantification of the proportion of Crispant fish with dysmorphic ISVs. Dysmorphic was defined as a vessel projection emerging from the ISV distinct from the central stalk. Error bars represent SEM. N, number of fish quantified unless indicated. *p < .05, ****p < .0001. ns, non-significant. All experiments were done at minimum in triplicate