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Fig. 3 Abnormal myeloid cell expansion in Tg(hsp70:E2A-PBX1-EGFP) fish caused by proliferation and apoptosis perturbation. (A) Immunofluorescence double staining of Lcp1 and bromodeoxyuridine (BrdU) antibodies reveals a significant increase in myeloid cell proliferation in the caudal hematopoietic tissue (CHT) region of 5 days post-fertilization (dpf) Tg(hsp70:E2A-PBX1-EGFP) larvae (N=22) compared with the siblings (N=17). Lcp1/BrdU double-positive cells are indicated by white arrows. (A’) Statistical analysis of percentage of Lcp1+ BrdU+ cells in panel (A). The black asterisks indicate statistical difference (Student t tests, mean ± standard error of the mean; *P<0.05). (B) Co-staining of Lcp1 and transferase dUTP nick end labeling (TUNEL) was used to detect the apoptosis in the CHT region of 5 dpf Tg(hsp70:E2A-PBX1-EGFP) larvae (N=22) compared with the siblings (N=24). Lcp1/TUNEL double-positive cells are indicated by white arrows. (B’) Statistical analysis of percentage of Lcp1+ TUNEL+ cells in panel (B). The black asterisks indicate statistical difference (Student t tests, mean ± standard error of the mean; **P<0.01).