ZFIN Image: Jones et al., 2024, Figure 6

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Figure Caption

Figure 6

Fam83fa is targeted to the lysosome. (a) Representative confocal images showing HEK293T cells transfected with a plasmid containing tandem fluorescent proteins GFP/mCherry (empty vector, EV), tandem GFP/mCherry fluorescently tagged Fam83fa protein, with or without Bafilomycin A1 (Baf) and tandem GFP/mCherry fluorescently tagged truncated Fam83fa^1-500 protein as labelled. Scale bars = 20 µm. (b) Ratio of mCherry/GFP in all conditions shown in (a). Ratio of each biological repeat (independent transfection) is displayed normalized to empty vector control. Error bars = s.e.m. *¹p = 0.0203 and * ²p = 0.0330, Fisher’s LSD test, n.s. = not significant.

Acknowledgments

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