ZFIN Image: Meserve et al., 2024, Fig 4

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Figure Caption

Fig 4 Asymmetric spiral fiber input to Mauthner neurons in celsr3 mutants correlates with biased escapes.

A-D) Region of hindbrain with Mauthner and spiral fiber neurons from 5 dpf Tol-056:GFP larvae in (A) siblings with unbiased escapes (40–60% right/left C1-bend direction ratio; all categories >6 escapes over 20 stimuli), (B) celsr3 mutants with unbiased escapes, (C) celsr3 mutants that only made left C1-bends (right Mauthner predicted active), and (D) celsr3 mutants that only made right C1-bends (left Mauthner predicted active). Composite images are Z-projections with ventral range for anterior portion and dorsal range for posterior portion. Kv1.1 (left and middle panels) localizes to endogenous axon caps (brackets) and ectopic caps (red arrows in B-D) presumed to represent spiral fiber misprojections. α-Cx35 (left and right panels) labels spiral fiber synapses at endogenous (A-D) and ectopic (B-D) caps. White dashed brackets in (C,D) indicate the axon cap with reduced Kv1.1/Cx35 signal, which corresponds to the inactive Mauthner, based on escape bias. Scale bar = 20 μM.

Acknowledgments

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