|
Fig. 2 The effects of Ponatinib on heart function of zebrafish larvae. (A) 1 dpf Tg (cmlc2: GFP) transgenic zebrafish were treated with 0.1 % DMSO or indicated concentrations of Ponatinib for 3 days. The represented images of Ponatinib-induced abnormal heart in 4 dpf zebrafish larvae. (B) SV-BA distance of 4 dpf zebrafish (n=8). Data are represented as mean ± SEM. ***P < 0.005 vs. control group. (C) Atrial area of 4 dpf zebrafish (n=8). Data are represented as mean ± SEM. *P < 0.05 and ***P < 0.005 vs. control group. (D) Ventricular area of 4 dpf zebrafish (n=8). Data are represented as mean ± SEM. **P < 0.01 and ***P < 0.005 vs. control group. (E) Heart rate of 4 dpf zebrafish (n=10). Data are represented as mean ± SEM. ***P < 0.005 vs. control group. (F) Ventricular fractional shortening of 4 dpf zenrafish (n=10). Data are represented as mean ± SEM. *P < 0.05 and ***P < 0.005 vs. control group. (G) Representative images of apoptotic cells in the 4 dpf zebrafish heart as determined by TUNEL staining.