FIGURE 1

FIGURE 1

Gorab‐deficient long bones are more sensitive to TGF‐β inhibition than the Gorab‐expressing axial skeleton. (a) Treatment scheme for the Gorab^Prx1 mice. 4 weeks old Gorab^Prx1 mice were injected with 10 mg/kg body weight of either control IgG or 1D11 thrice a week by intraperitoneal injection for 8 weeks. (b) 3D rendering and (c) Representative microCT images showing the proximal tibia of control and Gorab^Prx1 mice treated with control IgG or 1D11. Scale bar = 500 μm. Quantitation of (d) percentage of trabecular bone volume (BV/TV); (e) comparison of rescue effects in vertebrae, tibia, and femur; (f) trabecular number (Tb.N); (g) trabecular thickness (Tb.Th); and (h) trabecular separation (Tb.Sp) in proximal tibia of control IgG and 1D11 treated control and Gorab^Prx1 mice (N = 7). Scanned and analyzed with Scanco μCT40 at 10 μm. Histomorphometric analysis at the proximal tibia secondary spongiosa in control and 1D11 treated Gorab^Prx1 mutants for (i) number of osteoblasts per bone perimeter (N.Ob/B.Pm); and (j) number of osteoclasts per bone perimeter (N.Oc/B.Pm). (N = 5–6). *p < 0.05, **p < 0.01, ***p < 0.001, ns, no statistically significant difference (unpaired Welch's t‐test).