ZFIN Image: Fung et al., 2024, Fig. 2.

Image

Figure Caption

Fig. 2.

Germ cells are retinoic acid responsive during early gonad development. (A-H) Representative confocal images of 12 (A-D) and 23 (E-H) dpf wild-type gonads in Tg(RARE:YFP) transgenics. (I-L) Representative confocal images of a 13 dpf wild-type gonad from a Tg(piwil1:EGFP) transgenic stained using anti-Crabp2a antibody. (A,E) YFP-labeled RA-responsive cells are green; anti-Ddx4 antibody labeled germ cells (GCs) are red. (I) EGFP-labeled piwil1⁺ GCs are green; Crabp2a-expressing cells are red. (A,E,I) DAPI-labeled nuclei are blue. (B,F) Grayscale Ddx4, (C,G) YFP, (J) EGFP, (K) Crabp2a and (D,H,L) DAPI. (A-D,I-L) Z-projections. (E-H) Single slices. Scale bars: 50 μm. Wild type: 12 dpf, n=3 larvae; 23 dpf, n=1 fish; 13 dpf, n=1 fish. Both gonads from each fish were imaged.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development