Figure 4

Figure 4

PFKM overexpression improves retinal phenotype but not photoreceptor cell death at 5 dpf. (a) Human PFKM expression was detected 5 days post-injection in chm^ru848 and wt zebrafish. (b) mRNA-injected chm^ru848 showed a more preserved photoreceptor layer (white asterisk) with better retinal lamination and a thicker RPE layer compared to the uninjected mutants (n = 9 zebrafish per group). Scale bar: 50 μm; scale bar zoom in: 20 μm. (c) Cell viability is unchanged after PFKM overexpression in wt and chm^ru848 zebrafish (n = 9 zebrafish per group). Scale bar = 50 μm. ONL: outer nuclear layer; INL: inner nuclear layer. (d,e) Expression of anti-apoptotic (d) and pro-apoptotic (e) markers were analysed using RT-qPCR at 5 dpf in zebrafish eyes, from uninjected and injected wt and chm^ru848. Data are expressed as mean ± SEM from n = 3 (with 20 eyes per group). Statistical significance was determined by one-way ANOVA. * p < 0.05; ** p < 0.005; **** p < 0.0001. “●”—wt; “▲”—wt + PFKM; “▼”—chm^ru848; “◆”—chm^ru848 + PFKM.