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Fig. 4 Analysis of hsp70 gene expression in response to heat shock (HS) in zebrafish embryos. Wild type zebrafish embryos are exposed to heat shock at 37oC for 1 h at 24 hpf. hsp gene expression was analyzed in the control and heat shocked embryos by in situ hybridization. Compared to the control groups (A, C, E, G, I), a strong upregulation of expression was detected for hsp70.1/2/l (B), hsp70.3 (D), hspa1b (F), hsc70 (H), and hspa8b (J) in the heat shock (HS) treated embryos. Strikingly, hspa1b gene showed a muscle-specific upregulation in response to the HS stress. Side view. Anterior?left, dorsal?up. (K?O) Data of qRT-PCR analysis show quantified changes of hsp70.1/2/l (K), hsp70.3 (L), hspa1b (M), hsc70 (N), and hspa8b (O) gene expression in heat shocked embryos compared with non-HS control. * indicates significant difference at the p-value < 0.05; ** indicates significant difference at the means p-value < 0.01; **** indicates significant difference at the p-value <0.0001. Scale bar = 100 ?m.