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Fig. 5 - Supplemental 3 Olfactory system development in foxd3 mutants. (A, B) In situ hybridisation for the NCC marker crestin on 32 hpf controls and foxd3 mutants. Arrowheads = crestin-positive NCC cluster at the OP/brain interface, which is absent in foxd3 mutants. (C, D). Images (frontal view, maximum projection) of representative OPs of Tg(omp:meYFP); foxd3 -/- mutants and control siblings. (C’, D’) Laminin immunostaining (1 z-section) on the same embryos. (E–H) OP dimensions and length of the axon bundle at 28 hpf in foxd3 mutants and control siblings (n=31 controls and n=13 mutants from two independent experiments). (I–L) OP dimensions and length of the axon bundle in foxd3 mutants and control siblings at 36 hpf (n=21 controls and n=4 mutants from two independent experiments). Unpaired, two-tailed t tests for E, I, J, L and Mann-Whitney tests for F, G, H, K.