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Fig. 7 Dync1h1-deficiency impairs cilium biogenesis. (A) PRC cilium biogenesis in WT and dync1h1−/− retinas at 5 dpf, detected by immunofluorescence for axonemal microtubules using anti-α-acetylated tubulin antibody; nuclei stained by DAPI. (B) PRC development in WT and dync1h1+/− retinas at indicated ages, with axonemal microtubules by anti-α-acetylated tubulin; nuclei stained by DAPI. The lengths of microtube were labeled with white vertical lines. (C) Quantitative analysis of microtubule length in WT and dync1h1+/− retinas across ages. +/−, dync1h1 heterozygote; −/−, dync1h1 homozygote. Scale bar = 50 µm. Data are shown as mean ± SD. *, P < 0.05; **, P < 0.01, n = 3 biologically independent samples per group. DAPI, 4′,6-diamidino-2-phenylin-dole; dpf, days post fertilization; PRC, photoreceptor cell; SD, standard deviation; WT, wild type.