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Fig. 2 Selenoprotein-N deficient zebrafish embryos present with impaired spontaneous contractions. Spontanous tail and trunk contractions of zebrafish embryos were recorded and quantified at 24 hpf in SelN homozygous KO selenoncl502 (N = 45 and 29), selenoncl503 (N = 43,48), and homozygous mutant (Mt) selenoncl504 (N = 131,116) and their correspodent WT controls. (A) Mean duration of spontaneous contractions, percent time of contraction activity, and total number of contractions are reported in each line. “*” = p < 0.05, “**” = p < 0.01, “***” = p < 0.001, “****” = p < 0.0001. (B) WT, heterozygous and homozygous embryos were observed for hatching activity and recorded up until 80 hpf (N = 41–100 WT, 92–134 heterozygous, and 56–93 homozygous mutant embryos per line)