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Fig. 5 Lack of Crhr1 affects thermal stress-mediated muscle energy metabolism. (A) Glucose concentration and (B) glycogen concentration measured in WT and crhr1−/− zebrafish epaxial muscle exposed to the novel tank test for 1 h at either 28°C or 33°C. (C) Transcript abundance of muscle gys1 and (D) pyg1, and (E) enzyme activity of hexokinase, (F) pyruvate kinase, and (G) lactate dehydrogenase measured in WT and crhr1−/− zebrafish epaxial muscle exposed to the novel tank test for 1 h at either 28°C or 33°C. Asterisks indicate a significant difference between treatment interaction (Two-way ANOVA, Holm–Sidak post hoc, *p < 0.05) or (G) main effects (Two-way ANOVA, *p < 0.05, **p < 0.01). Crhr1, corticotropin-releasing hormone receptor 1; gys1, glycogen synthase 1; KO, crhr1−/−; LDH, lactate dehydrogenase; pyg1, glycogen phosphorylase 1; WT, wildtype.