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Fig. 4 - Supplemental 1 Quality control analysis of single-cell RNA sequencing data. (A) Identification of the effective cell number of the sample. The blue line represents the effective cells corresponding to barcodes, while the gray line denotes the background noise. (B) The basic cellular metrics before and after filtering, including the total number of detected genes (nFeature_RNA), the total number of UMIs (nCount_RNA), and the percentage of reads mapped to mitochondrial genes (Percent.mito). (C) The scatter plot comparing the cellular metrics before and after filtering, showing the relationship between nCount_RNA and nFeature_RNA. The Pearson correlation coefficients are indicated above the graph.