|
Fig. 1 Metrns loss-of-function causes heart jogging/looping and visceral organ positioning defects. (A) 48 hpf triplMut zebrafish display no gross phenotypic defects compared to wild type (WT) embryos. (B) Examples of 48 hpf embryos showing mRNA expression of the heart marker myl7 and the different heart looping phenotypes (ventral view). (C) Quantification in percentage of heart looping phenotypes at 48 hpf in WT, triplMut, metrn-/-, metrnla-/-, metrnlb-/-, triplMut+/- embryos; displayed p-values compared to WT: ****p-value:<1.0e-5 for triplMut, ****p-value:<1.0e-5 for metrn-/-, ****p-value:<1.0e-5 for metrnla-/-, *p-value: 0.003 for metrnlb-/-, ns p-value: 0.49 for triplMut+/-, p-value compared to metrnlb-/-: *p-value: 0.034 for triplMut+/- (not displayed). (D) Example of 56 hpf embryos showing gata6 mRNA expression highlighting the different visceral organ positioning phenotypes (ventral view). (E) Quantification in percentage of visceral organ positioning phenotypes at 56 hpf in WT and triplMut embryos, ***p-value: 0.00034. In (B) L=left, R=right. In (D) L=liver, P=pancreas, I=intestine. Scale bars in (A): 3mm, (B): 100 μm, (D): 500 μm.