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Fig. 3 Metrns are expressed during early zebrafish development. (A) Expression patterns of metrn, metrnla, and metrnlb during early embryonic development from two-cell stage to 12 hpf. (9 hpf and 12 hpf dorsal view) (B) Confocal cross-section of the midline region of 12 and 14 hpf zebrafish embryos showing mRNA expression of the metrn (magenta) and metrnla (cyan) and their co-expression in the area of the Kupffer’s vesicle (KV, indicated by arrowheads) by HCR. (C) Metrn and sox32 are co-expressed by dorsal forerunner cells (DFCs) as shown by double fluorescence in situ hybridization against metrn (magenta) and sox32 (cyan) on 10 hpf wild type embryos. (D) Metrnla and sox32 are co-expressed by DFCs as shown by double fluorescence in situ hybridization against metrnla (magenta) and sox32 (cyan) on 10 hpf wild type embryos. Scale bars in (A): 500 μm, (B) 250 μm, (C) and (D): 50 μm. L=left, R=right.