ZFIN Image: Eggeler et al., 2025, Fig. 4

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Figure Caption

Fig. 4 - Supplemental 1 Metrns loss-of-function leads to DFC disorganization and migration defects. (A) Sox32 antisense riboprobe in situ hybridization revealing DFC misclustering in 9 hpf metrn-/- and metrnla-/- embryos. (B) Quantification in percentage of the number of embryos with DFC clustering defects at 9 hpf in wild type (WT) and several metrns mutant backgrounds (Fisher exact test, ***p-value: 1.5e-05 for metrn-/- vs. metrn+/-; ***p-value: 2.5e-26 for metrnla-/- vs. metrnla+/-; ***p-value: 1.2e-13 for metrn-/- x metrnla-/- vs. metrn+/- +/- metrnla+/-; ***p-value: 2.2e-18 for triplMut vs. ♀WT x ♂triplMut and ***p-value: 2.1e-13 for triplMut vs. ♀triplMut x ♂WT). Error bars indicate standard deviation. (C) tbxta and sox17 DFC specification markers are expressed in the absence of Metrn proteins, as shown by in situ hybridization on WT and triplMut at 8 and 10 dpf, respectively. Scale bars: in (A and C) 0.5 mm.

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