FIGURE 7

FIGURE 7

Delivery of translation‐blocking morpholino oligos (MO) targeting rs1a and rs1b mRNA results in significant downregulation of visual perception and camera‐type eye development at 96 h post‐fertilization (hpf), as well as impaired photoreceptor development. Following RNA sequencing, gene ontology (GO) enrichment analyses were performed. A: All significantly downregulated GO terms for biological processes (BP; blue bars) and molecular function (MF; red bars). No terms for cellular components were significantly enriched in differentially expressed genes (DEGs). n refers to the number of DEGs mapped to the term. B: All upregulated GO terms for cellular components (CC; green bars). MF and BP did not yield terms significantly enriched in DEGs. n refers to the number of DEGs mapped to the term. C: Heatmap of phototransduction‐related genes (derived from Kyoto Encyclopedia of Genes and Genomes [KEGG] pathway). Red and green colors indicate respectively down‐ and upregulation of the genes in MO compared to scrambled (SC). The scale ranges from strongly downregulated (fold change 0.18) to slightly upregulated (fold change 2.0). D: Immunofluorescent staining of blue cone pigment small‐wavelength sensitive opsin (OPN1SW) in 4 and 5 day‐old SC‐ and MO‐injected zebrafish. Padj = adjusted p‐value. Dpf = days post‐fertilization.