ZFIN Image: Kamei et al., 2026, Fig. 2.

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Figure Caption

Fig. 2.

Canonical Wnt signaling highlights the point of tubule invasion. (A,B) Canonical Wnt target genes lef1 (A) and wnt4 (B) are expressed in new nephron tubules (marked by EdU incorporation) at the tubule fusion junction with the distal tubule (DT; dotted outline) marked by Tg(lhx1a:eGFP) expression. (C) The Tg(tcflef:dGFP) Wnt reporter transgene is expressed at the point of tubule fusion. (D,E) The canonical Wnt inhibitor IWR1 blocks formation of invasive basal protrusions (arrowheads). (F) Quantification of basal protrusion phenotype by Kappa curvature analysis in ImageJ reveals loss of protrusions after IWR1 treatment (Mary and Brouhard, 2019 preprint; see Materials and Methods). Green in B is autofluorescence. Blue fluorescence is Hoechst-stained nuclei. Scale bars: 5 µm. **P=0.0012 (Mann-Whitney test). Data are mean±s.d. (control: 0.6986±0.2750, n=9; IWR1 median: 0.2204±0.1136, n=7).

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Acknowledgments

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