Figure 2

Figure 2

Zebrafish PRL-3 knockdown impairs epiboly progression. Representative bright-field images showing developmental staging of zebrafish embryos injected with 4 ng control morpholino (MO) (N = 88) or zfPRL-3 MO (N = 60) and examined at the indicated time points. Percentages indicate the proportion of embryos in each group that were scored as the indicated developmental stage at each time point. (A–E) Control MO–injected embryos progressed normally: (A) 1k-cell stage (3 h post fertilization; hpf), (B) sphere (4 hpf), (C) dome (5 hpf), (D) shield (6 hpf), and (E) ~75% epiboly (8 hpf). (F–J) zfPRL-3 morphants showed delayed progression, remaining at earlier stages at later time points: embryos were at the 1k-cell stage at 3 hpf (F), remained at the sphere stage at 4–5 hpf (G,H), and were only at the dome stage at 6–8 hpf (I,J). Scale bars = 250 μm.