ZFIN Image: Xu et al., 2026, Figure 5

Image

Figure Caption

Figure 5

Notch deficiency mediates arterial valve defects in bmp16 mutants. (A) WISH analysis of notch1b expression in wild-type and bmp16 knockout embryos at 72 hpf. Scale bar: 100 μm. (B) qRT-PCR analysis of notch1b in adult hearts of wild-type and bmp16 knockout zebrafish. The statistical analysis was performed using unpaired Student’s t-test. (C) WISH analysis of notch1b expression in vehicle- or LDN-193189-treated embryos at 72 hpf and 96 hpf. Scale bar: 100 μm. (D) qRT-PCR analysis of notch1b in dissected embryonic hearts at 96 hpf following vehicle or LDN-193189 treatment. The statistical analysis was performed using unpaired Student’s t-test. (E) Schematic representation of the timeline for Yhhu-3792 treatment. (F) Time-lapse LSFM images of Tg(gata1:DsRed) embryos in wild-type or bmp16 knockout background treated with DMSO or Yhhu-3792 at 96 hpf. Individual blood cells were pseudocolor-coded for tracking. Scale bar: 20 µm. (G) Quantification of blood cells regurgitating from the bulbus arteriosus into the ventricle in wild-type and bmp16 knockout embryos treated with DMSO or Yhhu-3792. The statistical analysis was performed using two-way ANOVA. n = 7–11 per group. *, p < 0.05; **, p < 0.01; ****, p < 0.0001.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.