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Figure 3
Physical interactions between cdhr1a and pcdh15b can facilitate cell-cell adhesion.
(A) Immunoprecipitation of cdhr1a-FLAG and pcdh15b-MYC performed in HEK293 cell lysates. Pull down of cdhr1a-FLAG using anti-FLAG antibody beads also pulls down pcdh15b-MYC, while pull down of pcdh15b-MYC using anti-MYC antibody beads also pulls down cdhr1a-FLAG. (B) Diagrammatic representation of the K562 cell assay for assessing homophilic or heterophilic interactions between cadherins. (C) Brightfield microscopy analysis of K562 cell aggregation after transfection with either cdhr1a or pcdh15b or after co-culture of the two transfected populations. Magenta boxes indicate regions enlarged in the insets. (D) Quantification of the total number of aggregates observed in a single field of view. (E) Quantification of the number of average number of cells in each observed aggregate.