Figure 4

Figure 4 Construction and confirmation of the cdhr1a^-/- line.
(A) Exon/intron diagram of cdhr1a. The intron 6-exon 6-intron 7 junction is highlighted and the approximate location of the crRNA (black arrows) is depicted. (B) Genomic nucleotide sequence from the cdhr1a^Δ173 line highlighting the sequence location of the 173 bp deletion compared to WT sequence. (C) Amino acid sequence alignment between wildtype and the cdhr1a^Δ173 line. Alignment indicates that the 173 bp deletion leads to a frameshift and an immediate premature stop codon at AA146. (D) Diagrammatic representation of the domain structure of WT cdhr1a protein vs the cdhr1a^Δ173 allele. EC = cadherin domain, TD = transmembrane domain, CD = cytoplasmic domain. (E) Confocal microscopy of 5 dpf retinal cryosections from WT and cdhr1a^-/- retinas stained with anti-cdhr1a antibody (green), PNA (red-green cones - magenta) and WGA (blue cones - teal). Cdhr1a signal is detected along photoreceptor outer segments in the wildtype and completely missing in cdhr1a^-/-. ROS = rod outer segment, RIS = rod inner segment. Scale bar = 5 μm.