Fig. 3.

Fig. 3.

The lack of actinotrichia leads to morphological defects in the adult fin. (A,B) Bright-field images of live (A) and Alizarin Red-stained (B) adult wild-type sibling (n=8 fish) and double mutant fins (n=8 fish) at 10 months post-fertilization ranging between 28 mm and 30 mm standard length (SL). Double mutant dorsal and caudal fins are also shown in Fig. S7B1,B2. Scale bars: 1 mm. (C,D) Mean (+) lengths (C) and widths (D) of fins in wild-type siblings (n=7 fish) and double mutants (n=12 fish) at 140 dpf as a percentage of SL, ranging between 29 and 33 mm SL. Length and width were measured at the longest and widest points of each fin, respectively. (E,F) Number of rays (E) and percentage of bifurcating rays (F) in each fin in wild-type siblings (n=8 fish) and double mutants (n=8 fish) at 10 months post-fertilization ranging between 28 and 30 mm SL. *P<0.05 (unpaired two-tailed Mann–Whitney U-test, FDR=1%). Box plots show means (+), first and third quartiles (box), and lowest and highest values (whiskers).