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Figure S4.

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Gillotay et al., 2026 - The role of Nrf2 in thyroid maturation and hormone synthesis in vertebrate models
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Figure S4. Tg(tg:nrf2aT2A_mKO2nls) embryos overexpress nrf2a mRNA in their thyroid glands.
(A, B) Detection of nrf2a mRNA using whole-mount in situ hybridization on 72 hpf embryos. Analysis of nrf2a expression pattern shows the presence of nrf2a mRNA in a location corresponding to the developing thyroid gland in Tg(tg:nrf2aT2A_mKO2nls) embryos but not in WT embryos. (A, B, A′, B′) show a higher magnification of the thyroid region (dashed line square in pictures (A, B)) of the corresponding embryo. White arrows highlight the nrf2a mRNA in the developing thyroid. All images are ventral views, anterior to the left.

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