PUBLICATION
Distinct requirements for zebrafish angiogenesis revealed by a VEGF-A morphant
- Authors
- Nasevicius, A., Larson, J., and Ekker, S.C.
- ID
- ZDB-PUB-001220-6
- Date
- 2000
- Source
- Yeast (Chichester, England) 17(4): 294-301 (Journal)
- Registered Authors
- Ekker, Stephen C., Larson, Jon D., Nasevicius, Aidas
- Keywords
- zebrafish; VEGF-A; fli-1; flk-1; angiogenesis; vasculature; morpholino; morphant
- MeSH Terms
-
- Receptor Protein-Tyrosine Kinases/genetics
- Receptor Protein-Tyrosine Kinases/metabolism
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism
- Receptors, Growth Factor/genetics
- Receptors, Growth Factor/metabolism
- Signal Transduction
- Phenotype
- Blood Vessels/embryology*
- Proto-Oncogene Protein c-fli-1
- Gene Targeting*
- Animals
- Proto-Oncogene Proteins*
- Vascular Endothelial Growth Factor A
- Zebrafish/embryology*
- Zebrafish/genetics*
- Oligonucleotides, Antisense
- Neovascularization, Physiologic*
- Trans-Activators/genetics
- Trans-Activators/metabolism
- Embryonic Development
- Receptors, Vascular Endothelial Growth Factor
- Morpholines
- Mutation
- Gene Expression
- Body Patterning
- Endothelial Growth Factors/genetics*
- Endothelial Growth Factors/physiology*
- PubMed
- 11119306 Full text @ Yeast
Citation
Nasevicius, A., Larson, J., and Ekker, S.C. (2000) Distinct requirements for zebrafish angiogenesis revealed by a VEGF-A morphant. Yeast (Chichester, England). 17(4):294-301.
Abstract
Angiogenesis is a fundamental vertebrate developmental process that requires signalling by the secreted protein vascular endothelial growth factor-A (VEGF-A). VEGF-A functions in the development of embryonic structures, during tissue remodelling and for the growth of tumour-induced vasculature. The study of the role of VEGF-A during normal development has been significantly complicated by the dominant, haplo-insufficient nature of VEGF-A-targeted mutations in mice. We have used morpholino-based targeted gene knock-down technology to generate a zebrafish VEGF-A morphant loss of function model. Zebrafish VEGF-A morphant embryos develop with an enlarged pericardium and with major blood vessel deficiencies. Morphological assessment at 2 days of development indicates a nearly complete absence of both axial and intersegmental vasculature, with no or reduced numbers of circulating red blood cells. Molecular analysis using the endothelial markers fli-1 and flk-1 at 1 day of development demonstrates a fundamental distinction between VEGF-A requirements for axial and intersegmental vascular structure specification. VEGF-A is not required for the initial establishment of axial vasculature patterning, whereas all development of intersegmental vasculature is dependent on VEGF-A signalling. The zebrafish thus serves as a quality model for the study of conserved vertebrate angiogenesis processes during embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping