PUBLICATION
Eliminating zebrafish Pbx proteins reveals a hindbrain ground state
- Authors
- Waskiewicz, A.J., Rikhof, H.A., and Moens, C.B.
- ID
- ZDB-PUB-021107-1
- Date
- 2002
- Source
- Developmental Cell 3(5): 723-733 (Journal)
- Registered Authors
- Moens, Cecilia, Rikhof, Holly, Waskiewicz, Andrew
- Keywords
- none
- MeSH Terms
-
- Neurons
- Homeodomain Proteins/genetics
- Homeodomain Proteins/physiology*
- Rhombencephalon/embryology*
- Animals
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Mosaicism
- Base Sequence
- DNA-Binding Proteins*
- Molecular Sequence Data
- DNA, Complementary
- PubMed
- 12431378 Full text @ Dev. Cell
Citation
Waskiewicz, A.J., Rikhof, H.A., and Moens, C.B. (2002) Eliminating zebrafish Pbx proteins reveals a hindbrain ground state. Developmental Cell. 3(5):723-733.
Abstract
The vertebrate hindbrain is divided into serially homologous segments, the rhombomeres (r). Pbx and Hox proteins are hypothesized to form heterodimeric, DNA binding transcription complexes which specify rhombomere identities. Here, we show that eliminating zebrafish Lzr/Pbx4 and Pbx2 function prevents hindbrain segmentation and causes a wholesale anterior homeotic transformation of r2-r6, to r1 identity. We demonstrate that Pbx proteins interact with Hox paralog group 1 proteins to specify segment identities broadly within the hindbrain, and that this process involves the Pbx:Hox-1-dependent induction of Fgf signals in r4. We propose that in the absence of Pbx function, r2-r6 acquire a homogeneous ground state identity, that of r1, and that Pbx proteins, functioning primarily with their Hox partners, function to modify this ground state identity during normal hindbrain development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping