PUBLICATION
Reptin and pontin antagonistically regulate heart growth in zebrafish embryos
- Authors
- Rottbauer, W., Saurin, A.J., Lickert, H., Shen, X., Burns, C.G., Wo, Z.G., Kemler, R., Kingston, R., Wu, C., and Fishman, M.
- ID
- ZDB-PUB-021204-1
- Date
- 2002
- Source
- Cell 111(5): 661-672 (Journal)
- Registered Authors
- Fishman, Mark C., Rottbauer, Wolfgang
- Keywords
- none
- MeSH Terms
-
- Sequence Homology, Amino Acid
- beta Catenin
- Enzyme Activation
- Conserved Sequence
- Nuclear Proteins/genetics
- Nuclear Proteins/physiology*
- Trans-Activators/physiology
- Carrier Proteins
- Cytoskeletal Proteins/physiology
- ATP-Dependent Proteases
- Amino Acid Sequence
- Adenosine Triphosphatases/chemistry
- Adenosine Triphosphatases/physiology
- Heat-Shock Proteins/physiology
- Cardiomegaly/embryology
- Cardiomegaly/etiology
- Cardiomegaly/genetics
- Molecular Sequence Data
- Embryo, Nonmammalian
- Animals
- Cell Division
- Base Sequence
- Zebrafish
- Myocardium/cytology
- Myocardium/ultrastructure
- Heart/embryology*
- Gene Expression Regulation, Developmental
- Serine Endopeptidases/physiology
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Point Mutation
- PubMed
- 12464178 Full text @ Cell
Citation
Rottbauer, W., Saurin, A.J., Lickert, H., Shen, X., Burns, C.G., Wo, Z.G., Kemler, R., Kingston, R., Wu, C., and Fishman, M. (2002) Reptin and pontin antagonistically regulate heart growth in zebrafish embryos. Cell. 111(5):661-672.
Abstract
Organ size is precisely regulated during development, but the control mechanisms remain obscure. We have isolated a mutation in zebrafish, liebeskummer (lik), which causes development of hyperplastic embryonic hearts. lik encodes Reptin, a component of a DNA-stimulated ATPase complex. The mutation activates ATPase activity of Reptin complexes and causes a cell-autonomous proliferation of cardiomyocytes to begin well after progenitors have fashioned the primitive heart tube. With regard to heart growth, beta-catenin and Pontin, a DNA-stimulated ATPase that is often part of complexes with Reptin, are in the same genetic pathways. Pontin reduction phenocopies the cardiac hyperplasia of the lik mutation. Thus, the Reptin/Pontin ratio serves to regulate heart growth during development, at least in part via the beta-catenin pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping