PUBLICATION
nil per os encodes a conserved RNA recognition motif protein required for morphogenesis and cytodifferentiation of digestive organs in zebrafish
- Authors
- Mayer, A.N. and Fishman, M.C.
- ID
- ZDB-PUB-030728-19
- Date
- 2003
- Source
- Development (Cambridge, England) 130(17): 3917-3928 (Journal)
- Registered Authors
- Fishman, Mark C., Mayer, Alan
- Keywords
- none
- MeSH Terms
-
- Endoderm/physiology
- RNA/metabolism*
- Cell Differentiation/genetics
- Cell Differentiation/physiology
- Animals
- Amino Acid Motifs/genetics
- Amino Acid Motifs/physiology
- Digestive System/embryology*
- Liver/embryology
- Cloning, Molecular
- RNA-Binding Proteins/genetics*
- RNA-Binding Proteins/metabolism
- Zebrafish/embryology*
- Pancreas/embryology
- Amino Acid Sequence
- Molecular Sequence Data
- Epithelium/embryology
- PubMed
- 12874115 Full text @ Development
Citation
Mayer, A.N. and Fishman, M.C. (2003) nil per os encodes a conserved RNA recognition motif protein required for morphogenesis and cytodifferentiation of digestive organs in zebrafish. Development (Cambridge, England). 130(17):3917-3928.
Abstract
Digestive organ development occurs through a sequence of morphologically distinct stages, from overtly featureless endoderm, through organ primordia to, ultimately, adult form. The developmental controls that govern progression from one stage to the next are not well understood. To identify genes required for the formation of vertebrate digestive organs we performed a genetic screen in zebrafish. We isolated the nil per os (npo) mutation, which arrests morphogenesis and cytodifferentiation of the gut and exocrine pancreas in a primodial state. We identified the npo gene by positional cloning. It encodes a conserved protein, with multiple RNA recognition motifs, that is related to the yeast protein Mrd1p. During development npo is expressed in a dynamic fashion, functioning cell autonomously to promote organ cytodifferentiation. Antisense-mediated knockdown of npo results in organ hypoplasia, and overexpression of npo causes an overgrowth of gastrointestinal organs. Thus, npo is a gene essential for a key step in the gut morphogenetic sequence.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping